Part:BBa_K2587029:Design

P_j23102_RBS_ddh_T

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 422
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 390
  Illegal NgoMIV site found at 615
  Illegal AgeI site found at 825
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We performed a mutagenesis of recognition sites from the restriction enzymes BsaI and BbsI in the coding sequence of ddh.

Source

The gene ddh was isolated from the genome of Corynebacterium glutamicum and the other parts are taken from the CIDAR MoClo toolbox.